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http://hdl.handle.net/10867/139
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Title: | Tribulus terrestrisAlters the Expression of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 in Rabbit Ovaries of Mothers and F1 Female Offspring |
Authors: | Abadjieva, Desislava Kistanova, Elena |
Keywords: | Oocytes Ovaries Rabbits Cell cycle and cell division Gene expression RNA extraction Diet Granulosa cells ReProForce |
Issue Date: | 29-Feb-2016 |
Publisher: | PLOS ONE |
Citation: | Abadjieva D, Kistanova E (2016)Tribulus terrestrisAlters the Expression of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 in Rabbit Ovaries of Mothers and F1 Female Offspring. PLoS ONE 11(2): e0150400. |
Abstract: | Although previous research has demonstrated the key role of the oocyte-derived factors,
bone morphogenetic protein (BMP) 15 and growth differentiation factor (GDF) 9, in follicular
development and ovulation, there is a lack of knowledge on the impact of external factors,
which females are exposed to during folliculogenesis, on their expression. The present
study investigated the effect of the aphrodisiacTribulus terrestrison the GDF9 and BMP15
expression in the oocytes and cumulus cells at mRNA and protein levels during folliculogen
esis in two generations of female rabbits. The experiment was conducted with 28New Zea
land rabbits. Only the diet of the experimental mothers group was supplemented with a dry
extract ofT.terrestrisfor the 45 days prior to insemination. The expression of BMP15 and
GDF9 genes in the oocytes and cumulus cells of mothers and F1 female offspring was ana
lyzed using real-time polymerase chain reaction (RT-PCR). The localization of the GDF9
and BMP15 proteins in the ovary tissues was determined by immunohistochemical analy
sis. The BMP15 and GDF9 transcripts were detected in the oocytes and cumulus cells of
rabbits from all groups.T.terrestriscaused a decrease in the BMP15 mRNA level in the
oocytes and an increase in the cumulus cells. The GDF9 mRNA level increased significantly
in both oocytes and cumulus cells. The downregulated expression of BMP15 in the treated
mothers’ oocytes was inherited in the F1 female offspring born to treated mothers. BMP15
and GDF9 show a clearly expressed sensitivity to the bioactive compounds ofT.terrestris. |
URI: | http://hdl.handle.net/10867/139 |
Appears in Collections: | FP7-REGPOT
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